Soghigian, J.; Morinaga, G.; Yeo, H.; Wilkerson, R.; Linton, Y.-M.; Sallum, M. A.; Sharakov, I.; Sharakova, M.; Laurito, M.; Bang, W. J.; Shin, S.; Snyman, L.; Zavortink, T.; Sither, C.; Reiskind, M.; Wiegmann, B.

Mosquitoes are classified into two subfamilies, each monophyletic, and typically considered to both be ancient, having diverged more than 100 million years ago based on previous divergence analyses. A recent publication challenged this view with phylogenomic results primarily from the third codon position and UCEs. Utilizing alternative fossil placement and these phylogenomic data, these authors find that the Culicidae and Chaoboridae diverged in the lower Cretaceous, and that one mosquito subfamily, the Anophelinae, is nested within the Culicinae. These results are in stark contrast to previous results from diverse data sources, ranging from other genomic data, to morphology, to fossils. Here, we briefly detail the substantial evidence that supports two monophyletic subfamilies of extant mosquitoes, along with fossil evidence that supports the ancient divergence of these lineages.