Stephens, E.; Hamza, A.; Driessen, M. R. M.; O'Neil, N. J.; Stirling, P. C.; Hieter, P.

The cohesin complex has conserved roles in sister chromatid cohesion, DNA replication, genome organization, and the DNA damage response. We heterologously expressed the human cohesin complex in yeast to probe the behaviour of human cohesin. Human cohesin was unable to complement loss of function mutations in yeast cohesin, either as single subunits or as complexes, including in the context of co-expressing up to 12 human cohesin-associated genes. Heterologous expression of human cohesin in yeast expressing wildtype yeast cohesin resulted in dominant cohesion dysregulation and DNA damage sensitivity phenotypes. We used co-immunoprecipitation to demonstrate that human SMC proteins interact with endogenous yeast cohesin rings creating dominant-negative hybrid complexes that disrupt endogenous cohesin biology.