Stanurova, J.; Cermakova, K.; Zakova, L.; Sacha, P.; Jiracek, J.; Konvalinka, J.

Insulin is a key hormone in glucose homeostasis. Its lack causes severe health complications and has to be compensated by regular administration of insulin. Despite intense long-lasting research, a more stable substitute has yet to be discovered to alleviate patients\' issues. Here we report the development of a novel assay for screening potential insulin analogues based on the recently published method DIANA. Our assay meets the need for a fast, non-radioactive method as a sensitive alternative to the commonly used radioactive immunoassay.