Devos, H.; Mina, I. K.; Paradeisi, F.; Makridakis, M.; Tserga, A.; Mokou, M.; Zoidakis, J.; Mischak, H.; Vlahou, A.; Latosinska, A.; Roubelakis, M. G.

Collagen type I (COL(I)) is a key component of the extracellular matrix (ECM) and is involved in cell signalling and migration through interactions with cell receptors. Col-lagen degradation produces bioactive peptides (matrikines), which can influence cel-lular processes. In this study, we investigated the biological effects of nine most abun-dant, naturally occurring urinary COL(I)-derived peptides on human endothelial cells at physiological concentrations, using cell migration assays, mass spectrometry-based proteomics, flow cytometry, and AlphaFold3. While none of the peptides significantly altered endothelial migration by themselves, full-length COL(I) increased cell migra-tion, which was selectively inhibited by COL(I)-derived Peptide 1 (229NGDDGEAGKPGRPGERGPpGp249). This peptide uniquely contains the DGEA and GRPGER motifs, known to interact with integrin 2{beta}1. Flow cytometry confirmed the presence of integrin 2{beta}1, and AlphaFold3 modelling predicted an interaction between Peptide 1 and integrin 2{beta}1. Mass spectrometry-based proteomics investigating sig-nalling pathways revealed that COL(I) triggered phosphorylation events linked to in-tegrin 2{beta}1 activation and cell migration, which were absent in COL(I) plus peptide 1-treated cells. These findings identify Peptide 1 as a biologically active COL(I)-derived peptide capable of modulating collagen-induced cell migration, and provide a founda-tion for further investigation into its mechanisms of action and its role in urine excre-tion.