Yang, S.; Chen, M.; Craig, J. M.; Laszlo, A. H.; Gundlach, J. H.; Kaplan, C. D.; Ebright, R. H.

Single-molecule picometer-resolution nanopore tweezers (SPRNT) enables monitoring of translocation of a nucleic-acid motor protein on a nucleic-acid track with sequence registration, sub-nucleotide spatial resolution, sub-millisecond temporal resolution, and the ability to apply forces that assist or oppose translocation. Recently, we used SPRNT to analyze the translocation of single molecules of Escherichia coli RNA polymerase relative to the DNA template strand during transcription elongation, and we directly detected sequence-dependent pausing and formation of a "half-translocated state" at the E. coli yrbL consensus pause element. Here, we apply SPRNT to analyze the translocation of single molecules of yeast RNA polymerase II (Pol II) relative to the DNA template strand during transcription elongation with single-nucleotide spatial resolution and millisecond-scale temporal resolution at biologically relevant, saturating substrate concentrations; we compare translocation by intact, 12-subunit Pol II to translocation by a 10-subunit Pol II sub-assembly lacking the dissociable Rpb4-Rpb7 Pol II "stalk"; and we assess possible pausing by intact Pol II and stalk-less Pol II at the E. coli yrbL consensus pause element. The results show that intact Pol II elongates more rapidly than stalk-less Pol II and show that neither intact Pol II nor stalk-less Pol II pauses at the E. coli yrbL consensus pause element.