Grein, S.; Elschner, T.; Kardinal, R.; Bruder, J.; Strohmeyer, A.; Gunasekaran, K.; Witt, J.; Hermannsdottir, H.; Behrens, J.; U-Din, M.; Yu, J.; Heldmaier, G.; Schreiber, R.; Rozman, J.; Heine, M.; Scheja, L.; Worthmann, A.; Heeren, J.; Wachten, D.; Wilhelm-Juengling, K.; Pfeifer, A.; Hasenauer, J.; Klingenspor, M.

Indirect calorimetry is a cornerstone technique for metabolic phenotyping of animal models in preclinical research, with well-established experimental protocols and platforms. However, a flexible, extensible, and user-friendly software suite that enables standardized integration of data and metadata from diverse metabolic phenotyping platforms - followed by unified statistical analysis and visualization - remains absent. We present Shiny-Calorie, an open-source interactive web application for transparent data and metadata integration, comprehensive statistical data analysis, and visualization of indirect calorimetry datasets. Shiny-Calorie is compatible with data formats from widely used commercial metabolic phenotyping platforms, such as TSE and Sable Systems, and includes functionality for exporting processed data in these formats. Built using GNU R and a Shiny-based reactive interface, Shiny-Calorie enables intuitive exploration of complex, multi-modal longitudinal datasets comprising categorical, continuous, ordinal, and count variables. The platform incorporates state-of-the-art statistical methods for robust hypothesis testing, thereby facilitating biologically meaningful interpretation of energy metabolism phenotypes, including resting metabolic rate and energy expenditure. Overall, Shiny-Calorie streamlines routine analysis workflows and enhances reproducibility and transparency in metabolic phenotyping studies.